Fig. 4

Weighted linear regression and non-linear regression avoid the risk of obtaining biased kinetic parameters. a Using the data given in Table 2, the double-reciprocal plots obtained without weights (red line) and with weights (blue line) are shown. The inset focus on the points with higher weights in the weighted regression analysis. b The function dir.MM() from the renz package also allows for direct non-linear regression analysis. The curve and parameters obtained using this function are shown in this plot