Fig. 3

NODeJ analyses of two datasets from mutants (crwn1/2 and kaku4 crwn1/4) known to alter chromatin organization in A. thaliana cotyledon epidermis. A. Z-projection of guard cell nuclei (diploid cell) of wild type and crwn1/2 mutant leaf epidermis, stained with Hoechst as well as the NODeJ image result (scale bar 2 μm³). crwn1/2 mutants (n=39) and wild type plants (n=38) from Poulet et al. [8] (Additional file 1). B. Number of chromocenters. C. Mean chromocenter volume per nucleus. D. Mean distance from chromocenter border to the nuclear envelope per nucleus. E. Z-projection of epidermis (diploid and polyploid cells) of wild type and kaku4 crwn1/4 mutant, stained with Hoechst as well as the NODeJ image result (scale bar 2 μm³). kaku4 crwn1/4 triple mutant (n=851) and wild type plants (n=609) from Dubos et al. [9] (Additional file 1). F. Number of chromocenters. G. Mean chromocenter volume per nucleus. H. Mean distance from chromocenter border to the nuclear envelope per nucleus. Mann-Whitney U test P-value: * \(\le\)0.05, *** \(\le\)0.001. Box plots and statistical tests were made using various R packages [17, 18, 21] (Additional file 2 and 3 describe the computed parameters)