Fig. 4
From: NODeJ: an ImageJ plugin for 3D segmentation of nuclear objects
NODeJ 180bp repeat DNA FISH signal detection in cotyledon epidermis nuclei of A. thaliana. Results from the analysis of DNA FISH experiments of kaku4 crwn1/4 triple mutants (n = 93) and wild type (n = 65) from the Dubos et al. [9] dataset (Additional file 1). A. Z-projection of 3D DNA FISH of a wild type pavement cell nucleus (scale bar 2 μm3). The boxplots show: B. the number of of 180bp repeat signals per nucleus, C. the mean 180bp repeat signal volume per nucleus, D. the mean distance from 180bp repeat signal border to the nuclear envelope. Mann-Whitney U test P-value: * \(\le\)0.05, *** \(\le\)0.001. Box plots and statistical tests were made using various R packages [17, 18, 21] (Additional files 2 and 3 describe the computed parameters)