Fig. 5
From: rprimer: an R/bioconductor package for design of degenerate oligos for sequence variable viruses
Evaluation of assay performance using 10 stool samples testing positive for norovirus GI. Top: Fluorescence data from RT-qPCR with Assay A (designed using rprimer), in comparison to a reference assay. Each sample was run in duplicate wells. Bottom: Gel picture following RT-PCR with Assay B (designed using rprimer). NTC: no template control. The intended amplicon size is 924 bp