Fig. 4
From: qRAT: an R-based stand-alone application for relative expression analysis of RT-qPCR data

Evaluation of the impact of inter-run calibration on sample WT C ref G of the MP dataset. The difference between the Cq values is 0.02 for the non-calibrated plates, and eliminated after inter-run calibration. Orange colour indicates that data is calibrated. Blueish colour shows data before calibration. Colour shades indicate that data is derived from different runs