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Table 2 Comparison of the data output from qRAT and qPCRSoft using the SP dataset

From: qRAT: an R-based stand-alone application for relative expression analysis of RT-qPCR data

qRAT qPCRSoft
Sample Gene \(\Delta\)Cq \(-\Delta\)Cq FC FC.sd \(-\Delta\)Cq FC FC.sd
C GOI 1 2.76 \(-2.76\) 1 0 \(-2.77\) 1 0
C GOI 2 10.76 \(-10.76\) 1 0 \(-10.77\) 1 0
C GOI 3 0.14 \(-0.14\) 1 0 \(-0.15\) 1 0
C GOI 4 3.85 \(-3.85\) 1 0 \(-3.86\) 1 0
C GOI 5 3.26 \(-3.26\) 1 0 \(-3.27\) 1 0
C GOI 6 3.19 \(-3.19\) 1 0 \(-3.2\) 1 0
C GOI 7 6.48 \(-6.48\) 1 0 \(-6.49\) 1 0
T GOI 1 \(-4.28\) 4.28 132.06 26 4.27 132.38 27.93
T GOI 2 8.62 \(-8.62\) 4.39 1.26 \(-8.63\) 4.40 1.27
T GOI 3 \(-5.89\) 5.89 65.46 12.6 5.88 65.34 12.60
T GOI 4 \(-4.46\) 4.46 316.82 63 4.46 318.00 70.90
T GOI 5 \(-3.34\) 3.34 97.34 26.84 3.34 97.40 20.10
T GOI 6 \(-4.94\) 4.94 279.17 52.42 4.93 279.25 50.72
T GOI 7 \(-1.71\) 1.71 292.54 57.34 1.71 293.28 51.36
  1. Mean values are displayed for the relative \(\Delta\)Cq method, where values of the treatment sample (T) and control sample (C) are normalized to the reference gene, and the relative \(\Delta \Delta\)Cq method, where fold change (FC) values are normalized to the reference gene and made relative to the calibrator C