Table 2 Comparison of the data output from qRAT and qPCRSoft using the SP dataset
From: qRAT: an R-based stand-alone application for relative expression analysis of RT-qPCR data
qRAT | qPCRSoft | |||||||
|---|---|---|---|---|---|---|---|---|
Sample | Gene | \(\Delta\)Cq | \(-\Delta\)Cq | FC | FC.sd | \(-\Delta\)Cq | FC | FC.sd |
C | GOI 1 | 2.76 | \(-2.76\) | 1 | 0 | \(-2.77\) | 1 | 0 |
C | GOI 2 | 10.76 | \(-10.76\) | 1 | 0 | \(-10.77\) | 1 | 0 |
C | GOI 3 | 0.14 | \(-0.14\) | 1 | 0 | \(-0.15\) | 1 | 0 |
C | GOI 4 | 3.85 | \(-3.85\) | 1 | 0 | \(-3.86\) | 1 | 0 |
C | GOI 5 | 3.26 | \(-3.26\) | 1 | 0 | \(-3.27\) | 1 | 0 |
C | GOI 6 | 3.19 | \(-3.19\) | 1 | 0 | \(-3.2\) | 1 | 0 |
C | GOI 7 | 6.48 | \(-6.48\) | 1 | 0 | \(-6.49\) | 1 | 0 |
T | GOI 1 | \(-4.28\) | 4.28 | 132.06 | 26 | 4.27 | 132.38 | 27.93 |
T | GOI 2 | 8.62 | \(-8.62\) | 4.39 | 1.26 | \(-8.63\) | 4.40 | 1.27 |
T | GOI 3 | \(-5.89\) | 5.89 | 65.46 | 12.6 | 5.88 | 65.34 | 12.60 |
T | GOI 4 | \(-4.46\) | 4.46 | 316.82 | 63 | 4.46 | 318.00 | 70.90 |
T | GOI 5 | \(-3.34\) | 3.34 | 97.34 | 26.84 | 3.34 | 97.40 | 20.10 |
T | GOI 6 | \(-4.94\) | 4.94 | 279.17 | 52.42 | 4.93 | 279.25 | 50.72 |
T | GOI 7 | \(-1.71\) | 1.71 | 292.54 | 57.34 | 1.71 | 293.28 | 51.36 |