Fig. 2

SEMplMe confirms differences in methylated SEM scores for previously classified sensitive versus insensitive transcription factors. A. Known methylation sensitive transcription factor MYC shows a large difference between methylated and unmethylated nucleotides at most positions. B. Known methylation insensitive transcription factor CEBPa shows very little difference between methylated and unmethylated nucleotides at most positions. For some positions (i.e. position 5), a small increase in binding is predicted for a methylated cytosine. C. Transcription factors previously annotated as methylation sensitive and insensitive show a significant difference in methylated (M/W) and non-methylated (C/G) SEM scores (T-test C-M p –value = 0.007 and G-W p-value = 1.32*10^–7). Error bars represent standard deviation