Fig. 4

SEMplMe predictions agree with in vitro experimental methods. A. SEMplMe agrees with previously published protein binding microarray (PBM) data of methylated and unmethylated binding sites for 8 transcription factors (R² = 0.67) [10]. B. SEMpl shows a reduced correlation with PBM data compared to SEMplMe (R² = 0.56), suggesting the addition of methylation data improves methylated sequence predictions. C. SEMplMe predictions correlate with previously published electrophoretic mobility shift assay (EMSA) data for methylated, hemi-methylated, and unmethylated binding sites for ATF4 and CEBPB (R² = 0.65) [9]. Grey ribbons represent Pearson’s 0.95 CI