Fig. 2

Statistics on simulated single-cell data with no trajectory. a Input simulated single-cell data. b Box plots of p-values obtained for each statistic on the dataset, with the median p-values measuring the overall statistical significance for the presence of trajectory. c–e are null distributions of three statistics obtained by permutation: c \(D_1\), number of degree-1 nodes, at \(k=16\), d \(D_2\), number degree-2 nodes, at \(k=8\), and e \(L_{\max }\), length of a longest path, at \(k=17\). The value of k for each statistic corresponds with the median p-value of the respective statistic. In each distribution, the observed test statistics are denoted by dashed orange lines