Skip to main content
Fig. 4 | BMC Bioinformatics

Fig. 4

From: Image segmentation and separation of spectrally similar dyes in fluorescence microscopy by dynamic mode decomposition of photobleaching kinetics

Fig. 4

Comparison of experimental and reconstructed bleach stacks of DHE in C. elegans. A, B montage of selected frames (i.e., frame (#1, #25, #50, #75 and #100) of the experimental fluorescence stack of DHE labeled C. elegans (left column) and of the reconstructed image stack obtained from the DMD of rank 5 (right column). Right column, absolute error between original stack and DMD reconstruction. The intensity range is identically scaled in 16-bit format. B, integrated intensity of original (blue symbols) and reconstructed image stacks (red symbols). C, mean intensity in color-coded boxes (see #50 in A for location of regions of interest, ROI) for original (red, yellow and cyan lines) and reconstructed video stacks (blue, green and pink lines). Note that intensities of the DMD reconstruction perfectly coincide with the intensities of the original stack; therefore, only the line colors of the reconstruction are visible. Bar, 20 µm. See text for further details

Back to article page