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Fig. 7 | BMC Bioinformatics

Fig. 7

From: Image segmentation and separation of spectrally similar dyes in fluorescence microscopy by dynamic mode decomposition of photobleaching kinetics

Fig. 7

DMD of C. elegans bleach stacks allows for discrimination of autofluorescence from DHE probe intensity. A, montage of individual dynamic modes shown as every second image of the corresponding image stacks. Dynamic mode 3 (‘DMD3’) resembles cellular autofluorescence of nematodes (upper row in A), while dynamic mode 4 and 5 (‘DMD4’ and ‘DMD5’) constitute DHE fluorescence (two middle rows in A). The sum of mode 4 and 5 shows the total DHE fluorescence (lower row in A). B–D, color overlay of mode decomposition with mode 3 resembling autofluorescence in red and sum of mode 4 and 5 representing DHE fluorescence in green. B, first frame, C, 10th frame and D, 20th frame of this color representation of the DMD, showing the rapid bleaching of DHE fluorescence (green) compared to autofluorescence (red). Bar, 20 µm

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BMC Bioinformatics

ISSN: 1471-2105

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