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Fig. 8 | BMC Bioinformatics

Fig. 8

From: Image segmentation and separation of spectrally similar dyes in fluorescence microscopy by dynamic mode decomposition of photobleaching kinetics

Fig. 8

DMD of bleach stacks of BHK cells labeled with two green probes, Alexa488-Tf and C6-NBD-SM. BHK cells were labeled with 4 µM C6-NBD-SM and with 20 µg/ml Alexa488-Tf, both emitting in green, as described in Materials and Methods. A, montage of selected frames (every 5th frame) of such double labeled cells. B, reconstruction of DMD of the bleach stack in A with the sum of dynamic modes 1, 3 and 4 shown in green (resembling the fast-bleaching C6-NBD-SM) and sum of dynamic mode 2 and 5 (resembling the slowly bleaching Alexa488-Tf) shown in red. Bar, 20 µm. C, mode amplitudes and D, eigenvalues of the DMD plotted on the unit circle

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BMC Bioinformatics

ISSN: 1471-2105

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