Image segmentation and separation of spectrally similar dyes in fluorescence microscopy by dynamic mode decomposition of photobleaching kinetics

Table 1 Quantification of segmentation accuracy of DMD for the three different image regions

Method	Ground truth 1 vs. mode 1	Ground truth 2 vs. mode 2	Ground truth 3 vs. mode 3
Triangle	J = 0.999	J = 0.996	J = 0.998
Triangle	D = 0.999	D = 0.998	D = 0.999
Minimum	J = 1.000	J = 1.000	J = 1.000
Minimum	D = 1.000	D = 1.000	D = 1.000
Isodata	J = 1.000	J = 1.000	J = 1.000
Isodata	D = 1.000	D = 1.000	D = 1.000
Li	J = 1.000	J = 1.000	J = 1.000
Li	D = 1.000	D = 1.000	D = 1.000
Mean	J = 1.000	J = 0.651	J = 0.606
Mean	D = 1.000	D = 0.789	D = 0.755
Otsu	J = 1.000	J = 1.000	J = 1.000
Otsu	D = 1.000	D = 1.000	D = 1.000
Yen	J = 0.995	J = 0.988	J = 0.988
Yen	D = 0.997	D = 0.994	D = 0.994

ISSN: 1471-2105