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Fig. 1 | BMC Bioinformatics

Fig. 1

From: PrimedSherlock: a tool for rapid design of highly specific CRISPR-Cas12 crRNAs

Fig. 1

Overview of CRISPR-Cas 12 based assay approaches. Firstly, a crRNA is complexed with a Cas12 enzyme in reaction buffer. Secondly, reporter oligos and sample RT-RPA or RT-PCR product are introduced to the reaction buffer. Thirdly, target sequence recognition causes enzyme activation and collateral cleavage of reporter ssDNA. Lastly, reporter molecules either FAM-Biotin or FAM-Quencher are respectively reported on via lateral flow or Fluorescence Assay

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BMC Bioinformatics

ISSN: 1471-2105

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