Fig. 6
From: PrimedSherlock: a tool for rapid design of highly specific CRISPR-Cas12 crRNAs
CRISPR-Cas12a Ultra was used to further validate PrimedSherlock crRNAs. Results of fluorescence detection assays for top-ranked primer and crRNA combinations. Fluorescence values represent the average of two biological replicates measured every minute for two hours. Controls assays were conducted without virus gRNA template (No Template), with two non-target strains per sample (Off-Target Flaviviruses), and for target samples without Reverse Transcriptase (-RT)