Table 1 A list of the top-scoring primer pairs and associated crRNA combinations generated from PrimedSherlock. Primer pairs and crRNAs were commercially synthesized (Integrated DNA Technologies) with /Alt1/ tags added to the crRNA for stability
From: PrimedSherlock: a tool for rapid design of highly specific CRISPR-Cas12 crRNAs
Viral Target | Strains Utilized | Primers 3’- > 5’ | crRNA Pairs 3’- > 5’ |
|---|---|---|---|
WNV | 2629 | F 5-TTGCTATTTGGCTACCGTCAGCGATCTC-3 R 5-TGGCGCATGTGTCAATGCTTCCTTTGCC-3 | crRNA-1 5-TTTGGCAAAGGAAGCATTGACACA-3 crRNA-2 5-TTTGTGTGCAGACAAGGAGTGGTG-3 |
ZIKV | 557 | F 5-CTGGAAACAGTCCCCGGCTCGATGTGGC-3 R 5-TCTTCACGTATACGTACCACGCTCCAGC-3 | crRNA-1 5-TTTCTCCCTGGTGGAGGATGACGG-3 crRNA-2 5-TTTGCAGCTGGAGCGTGGTACGTA-3 |
DENV1 | 2230 | F 5-GATGGCCAGTGTGGAGCCCCATTGGATA-3 R 5-CAGCCACATGGCCAATCCCTAAGTCTTT-3 | crRNA-1 5-TTTGTGGTTTCCAGCAGTCCCATC-3 crRNA-2 5-TTTGTTATTCATGATACTGACAGT-3 |
DENV2 | 776 | F 5-ACCTTAGCGACCGGGCCCATCTCCACAC-3 R 5-GTTTGTTGTGTTCTTCATGATGGAAAAG-3 | crRNA-1 5-TTTAGGGGGAGCTACTTGGCCGGA-3 crRNA-2 5-TTTCCATCATGAAGAACACAACAA-3 |
DENV3 | 946 | F 5-TGGGAGGAAGAGGCTGAGCAAACAGGAGTG-3 R 5-GGGTTTGCTTTTGCCAAGTATGCCAGACCA-3 | crRNA-1 5-TTTCCATACTCCATACCCGCAACA-3 crRNA-2 5-TTTGAAAACAGCATTACTAATAGT-3 |
DENV4 | 215 | F 5-AGTGTTCAAGATGTCACCAGGATACGTG-3 R 5-CTAAGGTTCCCACTTGGGTGTTGTCAAA-3 | crRNA-1 5-TTTCAATTCCACATGACCTCATGG-3 crRNA-2 5-TTTAAGGAAACTCACTTCAAGAGA-3 |