Fig. 4

Heatmap analysis and examples of significantly altered distribution profiles. A The normalized regression profiles for different treatment conditions for aphidicolin (A1–A4) and nocodazole (N1 and N4), as well as Luciferase are displayed. A heatmap showed the distribution of all cell populations with at least one significantly changed profile for the features 'Area', 'Mean Intensity DAPI' and 'Total Intensity DAPI' among the SOPRA cluster profiles. Wells treated with aphidicolin or nocodazole are displayed in shades of green or blue in the row sidebar. Wells Mock-treated or treated with siRNA against Luciferase or AllStars are indicated in red, orange and yellow, respectively. Wells treated with siRNA against specific genes are displayed in grey in the row sidebar. The heatmap is clustered using hierarchical clustering, and a dendrogram with a cut-off of 1.8 is performed, resulting in the heatmap groups (1), (2) and (3). The Venn diagram displays the distribution of the significantly changed profiles for each treatment among the heatmap groups (1–3). B Examples of profiles for the features ‘Area’, ‘Total Intensity DAPI’ and ‘Mean Intensity DAPI’ of cell populations significantly changed upon siRNA treatment, as well as the corresponding microscopic and FACS images