Fig. 4
From: INFLECT: an R-package for cytometry cluster evaluation using marker modality
INFLECT identifies additional heterogeneity present within labeled data. A-B tSNE embedding of 40,000 events from Levine32, in A colored for the 14 phenotypes as identified by Phenograph in the original paper. In B, 61 metaclusters identified through INFLECT. C Density plots of selected markers for CD16 + NK cells. X-axis denote Arcsinh(x/5) transformed expression values. D Density plots of selected markers highlighting heterogeneity within CD16 + NK cells, which was split into metaclusters 60 and 61. X-axis denote Arcsinh(x/5) transformed expression values. Metacluster 60 has a CD16-dim phenotype, whereas metacluster 61 has high CD16 expression