Fig. 4

Detecting moonlighting interactions using partial correlation. A Heatmap showing KRAS, NRAS, BRAF essentiality in selected cell lines. KRAS, NRAS, and BRAF are mutually exclusive, while RAF1 is essential in KRAS and NRAS backgrounds. B KRAS-NRAS-RAF1 moonlighting trio, with PCC and Partial correlation coefficients listed by the respective edges. Red edges indicate negative correlation coefficient, while blue edges indicate positive correlation coefficient. C CDK6-CDK4-CCND1 moonlighting trio, with correlation coefficients of edges listed. D IGF1R-MET-CPD moonlighting trio, with correlation coefficients of edges listed. E Network illustrating Ragulator complex, HOPS and CORVET complexes with shared subunits. Red colored edges indicate negative PCC, blue colored edges indicate boosted partial correlation coefficient, black colored edges are positive PCC. The yellow nodes depict genes recovered using partial correlation, orange nodes are genes recovered with PCC, and gray nodes depict genes annotated to be in the complex, that were not present in our top-ranking pairs