Fig. 2

Phenotype characterisation of EcN and validation of GEM. A Circular heatmap of phenotype microarray data. PM1 and PM2 contain carbon sources (blue ring), PM3 nitrogen sources (green ring), and PM4 phosphorus and sulfur (red ring). For each nutrient, an activity index was assigned between 0 (no growth, red) and 9 (maximum growth, green). Activities of 3 and above were considered as growth. B Comparison of the number of nutrients that enabled the growth of EcN in the work of Kim et al. [26] and in this work. The carbon (C) source when testing the nitrogen (N)/phosphorus (P)/sulfur (S) source was succinate/citrate and pyruvate, respectively. Total is the total number of sources tested for each nutrient. C Comparison of phenotype microarray data of EcN (Biolog) to growth predictions made by the final iHM1533 GEM (Model)