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Fig. 3 | BMC Bioinformatics

Fig. 3

From: High-quality genome-scale metabolic network reconstruction of probiotic bacterium Escherichia coli Nissle 1917

Fig. 3

Comparison of 13C fluxomics data to iHM1533 flux predictions on minimal media. Comparison of predicted internal fluxes to experimental 13C fluxomics data from Revelles et al. [25] of EcN on glucose (A) and gluconate (B). The Flux Variability Analysis (FVA) was run with FVA thresholds from 90 to 99%. The Parsimonious FBA (pFBA) is depicted as squares and the experimental data is visualised with the mean (dots) and standard deviation of three biological replicates (error bars, too small to observe) The flux is normalised to the glucose/gluconate uptake rate. The conversion of malate to oxaloacetate can both be performed by malate dehydrogenase (MDH) and by malate:FAD oxidoreductase (DMALRED) that uses FAD+ instead of NAD+. Therefore both reactions are included

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