Table 1 The final selected peptides of PspA and PhtD, and the mutated sequence of domain 4 of Ply included in the vaccine construct
Protein | Region | Peptide sequence | Position |
|---|---|---|---|
PspA | A Region | SPVASQSKAEKDYDAAVKKSEAAKKAYE | 3–30 (PspA2) |
VQQAYLAYQRASNKAEAAKMI | 74–94 (PspA2) | ||
B Region | LKEIDESDSEDYVKEGFRAPLQSELD | 182–207 (PspA1) | |
KDVEDFKNSDGEQAGQYLAAAEE | 266–288 (PspA1) | ||
LKEIDESESEDYAKEGFRAPLQSKLD | 190–215 (PspA2) | ||
DQLKAVEENNNVEDYSTEGLEK | 243–264 (PspA2) | ||
KLLDNLDPEGKTQDELDKEAAEA | 325–347 (PspA3) | ||
SNLEILLGGADPEDDTAALQNK | 368–389 (PspA3) | ||
KVLATLDPEGKTQDELDKEAAEA | 254–276 (PspA4) | ||
SKLEDNLKDAETNNVEDYIKEG | 297–318 (PspA4) | ||
PEGKTQDELDKEAAEDANIEALQNKVA | 258–284 (PspA5) | ||
RLQSDLKDAEENNVEDYVKEGL | 299–320 (PspA5) | ||
C Region | APKPEQPA | 354–361 (PspA1) 426–433 (PspA3) 365–372 (PspA5) | |
PhtD | C-terminal of PhtD | AAQAYAKEKGLTPPSTDHQDSGNTEAKGAEAIYNRVKAAKKVPLDRMPYNLQYTVEVKNGS | 559–619 |
KPQTEKPEEDKEHDEVSEPTHPESDEKENHVGLNPSA | 700–736 | ||
Ply | Domain 4 | NGDLLLDHSGAYVAQYYITWDELSYNHQGKEVLTPKAWDRNGQDLTAHFTTSIPLKGNVRNLSVKIREGTGLAFEWWRTVYEKTDLPLVRKRTISIWGTTLYPQVEDKVEND | 360–471 |