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Table 2 Physicochemical and immunological properties of the candidate vaccine

From: Immunoinformatics-aided design of a new multi-epitope vaccine adjuvanted with domain 4 of pneumolysin against Streptococcus pneumoniae strains

Molecular features

Prediction

No. of amino acids

590

Molecular weight (kDa)

62.96

Theoretical pI

4.60

Total no. of negatively charged residues (Asp + Glu)

115

Total no. of positively charged residues (Arg + Lys)

64

Estimated half-life

 > 10 h (Escherichia coli, in vivo)

 > 20 h (yeast, in vivo)

30 h (mammalian reticulocytes, in vitro)

Instability index

33.71 (Stable)

Aliphatic index

59.63

Grand average of hydropathicity (GRAVY)

− 1.028

Solubility (SOLpro)

0.958393 (Soluble)

Antigenicity (VaxiJen)

0.8833 (Probable ANTIGEN)

Antigenicity (ANTIGENpro)

0.850188 (Probable ANTIGEN)

Allergenicity (AlgPred/AllerTOP)

Non-allergen