Table 4 The predicted IFN-γ epitopes from the proposed construct. The hybrid approach (MERCI and SVM) were used to predict IFN-gamma epitopes by the IFNepitope server. The results revealed that there were 29 and 45 positive epitopes with scores greater or equal to 1 for the Ply4 adjuvant candidate and the main sequence of vaccine, respectively. The sequences were sorted from higher to lower scores
No. | Sequence | Method | Score | No. | Sequence | Method | Score |
|---|---|---|---|---|---|---|---|
The candidate adjuvant | |||||||
1 | AYVAQYYITWDELSY | MERCI | 5 | 16 | GTGLAFEWWRTVYEK | MERCI | 2 |
2 | YVAQYYITWDELSYN | MERCI | 5 | 17 | TGLAFEWWRTVYEKT | MERCI | 2 |
3 | VAQYYITWDELSYNH | MERCI | 5 | 18 | NGQDLTAHFTTSIPL | MERCI | 1 |
4 | AQYYITWDELSYNHQ | MERCI | 5 | 19 | GQDLTAHFTTSIPLK | MERCI | 1 |
5 | QYYITWDELSYNHQG | MERCI | 5 | 20 | QDLTAHFTTSIPLKG | MERCI | 1 |
6 | YYITWDELSYNHQGK | MERCI | 5 | 21 | DLTAHFTTSIPLKGN | MERCI | 1 |
7 | YITWDELSYNHQGKE | MERCI | 5 | 22 | LTAHFTTSIPLKGNV | MERCI | 1 |
8 | IREGTGLAFEWWRTV | MERCI | 3 | 23 | TAHFTTSIPLKGNVR | MERCI | 1 |
9 | REGTGLAFEWWRTVY | MERCI | 3 | 24 | AHFTTSIPLKGNVRN | MERCI | 1 |
10 | GAYVAQYYITWDELS | MERCI | 2 | 25 | RNLSVKIREGTGLAF | MERCI | 1 |
11 | LSVKIREGTGLAFEW | MERCI | 2 | 26 | NLSVKIREGTGLAFE | MERCI | 1 |
12 | SVKIREGTGLAFEWW | MERCI | 2 | 27 | GLAFEWWRTVYEKTD | MERCI | 1 |
13 | VKIREGTGLAFEWWR | MERCI | 2 | 28 | LAFEWWRTVYEKTDL | MERCI | 1 |
14 | KIREGTGLAFEWWRT | MERCI | 2 | 29 | AFEWWRTVYEKTDLP | MERCI | 1 |
15 | EGTGLAFEWWRTVYE | MERCI | 2 | Â | Â | Â | Â |
The main vaccine | |||||||
1 | TPPSTDHQDSGNTEA | MERCI | 3 | 24 | ESEDYAKEGFRAPLQ | MERCI | 2 |
2 | PPSTDHQDSGNTEAK | MERCI | 3 | 25 | SEDYAKEGFRAPLQS | MERCI | 2 |
3 | PSTDHQDSGNTEAKG | MERCI | 3 | 26 | EDYAKEGFRAPLQSK | MERCI | 2 |
4 | STDHQDSGNTEAKGA | MERCI | 3 | 27 | EQPAGPGPG | SVM | 1.08 |
5 | TDHQDSGNTEAKGAE | MERCI | 3 | 28 | PGPGVQQAYLAYQRA | SVM | 1.03 |
6 | DHQDSGNTEAKGAEA | MERCI | 3 | 29 | GLTPPSTDHQDSGNT | MERCI | 1 |
7 | LTPPSTDHQDSGNTE | MERCI | 2 | 30 | QDSGNTEAKGAEAIY | MERCI | 1 |
8 | HQDSGNTEAKGAEAI | MERCI | 2 | 31 | LDRMPYNLQYTVEVK | MERCI | 1 |
9 | KKSEAAKKAYEGPGP | MERCI | 2 | 32 | DRMPYNLQYTVEVKN | MERCI | 1 |
10 | KSEAAKKAYEGPGPG | MERCI | 2 | 33 | RMPYNLQYTVEVKNG | MERCI | 1 |
11 | SEAAKKAYEGPGPGV | MERCI | 2 | 34 | MPYNLQYTVEVKNGS | MERCI | 1 |
12 | EAAKKAYEGPGPGVQ | MERCI | 2 | 35 | NPSAGPGPGSPVASQ | MERCI | 1 |
13 | AAKKAYEGPGPGVQQ | MERCI | 2 | 36 | EDYVKEGFRAPLQSE | MERCI | 1 |
14 | AKKAYEGPGPGVQQA | MERCI | 2 | 37 | DYVKEGFRAPLQSEL | MERCI | 1 |
15 | PGLKEIDESESEDYA | MERCI | 2 | 38 | YVKEGFRAPLQSELD | MERCI | 1 |
16 | GLKEIDESESEDYAK | MERCI | 2 | 39 | VKEGFRAPLQSELDG | MERCI | 1 |
17 | LKEIDESESEDYAKE | MERCI | 2 | 40 | KEGFRAPLQSELDGP | MERCI | 1 |
18 | KEIDESESEDYAKEG | MERCI | 2 | 41 | EGFRAPLQSELDGPG | MERCI | 1 |
19 | EIDESESEDYAKEGF | MERCI | 2 | 42 | DPEDDTAALQNKGPG | MERCI | 1 |
20 | IDESESEDYAKEGFR | MERCI | 2 | 43 | PEDDTAALQNKGPGP | MERCI | 1 |
21 | DESESEDYAKEGFRA | MERCI | 2 | 44 | EDDTAALQNKGPGPG | MERCI | 1 |
22 | ESESEDYAKEGFRAP | MERCI | 2 | 45 | DDTAALQNKGPGPGK | MERCI | 1 |
23 | SESEDYAKEGFRAPL | MERCI | 2 | Â | Â | Â | Â |