Fig. 4

Features important for predicting expressibility in non-knottins are different from the features that are important for predicting expressibility in knottin peptides. This figure shows absolute SHAP values, which illustrate to what degree a feature affects prediction, calculated for different AlphaFold2 features. A The relative importance of amino acid position for prediction of expressibility in non-knottin and knottin peptides. In non-knottins, a region near the C-terminus (position 42 to 47) was identified as playing an important role in determining expressibility. In knottin peptides, the features at amino acid position 7–9 near the N-terminus were most important for expressibility. B The relative importance of the different AlphaFold2 representations for expressibility prediction of non-knottin and knottin peptides. For non-knottins, the structure representation module representation was most important for prediction of expressibility. On the other hand, in knottin peptides, the abstract single representation was most important for prediction of expressibility