Figure 3

Filtering results on the tomograms of stained specimens. (a) spiny dendrite; (b) algae chloroplast; (c) mitochondrion. The original tomograms (left), the results with TOMOBFLOW (centre), and the results with three iterations of the median filtering (right) are shown. Only a representative slice of the tomograms is presented. The number of iterations of TOMOBFLOW were (a) 150, (b) 150 and (c) 70. The datasets (a) and (b) were taken from the Cell Centered Database (accession codes 13 and 3408, respectively). The dataset (c) was kindly provided by Dr. G. Perkins (National Center for Microscopy and Imaging Research-NCMIR, UCSD, USA). In the three datasets, the results with TOMOBFLOW have the background particularly flat with respect to the original tomogram, and also with respect to the results with median filtering. Moreover, TOMOBFLOW outperforms the median filtering in the preservation of the structural features. The arrows point to areas where the sharpness of the features is especially apparent after the processing with TOMOBFLOW.