Table 1 Algorithms used in the QPCR web application
From: QPCR: Application for real-time PCR data management and analysis
Name | Description | Author | Cq | Efficiency |
|---|---|---|---|---|
Cy0 | The method operates on raw fluorescence data and fits a five parameter Richard's curve. Next it calculates the tangent of the inflection point and intersects it with the abscissa axis which results in the Cq value. | Guescini et al. [27] | ✓ | - |
LinReg | The method operates on background corrected data and uses log transformed values to construct a slope with the highest correlation to the original curve. The parameters of this slope are used to calculate the amplification efficiency. | Ramakers et al. [8] | - | ✓ |
Miner | The method operates on raw fluorescence data and fits a four-parameter logistic curve to determine the Cq value by using the second derivative maximum. The efficiency is calculated by using a weighted average of a fitted exponential curve. | Zhao et al. [28] | ✓ | ✓ |
RutledGene | The method operates on raw fluorescence data and fits a four-parametric sigmoid function to calculate efficiency values. | Rutledge et al. [29] | - | ✓ |
SoFar | The method operates on raw fluorescence data and fits exponential or sigmoid function on smoothed data to calculate Cq and efficiency values. | Wilhelm et al. [30] | ✓ | ✓ |
TAQ | The method operates on raw fluorescence data and performs a linear regression on log transformed data to determine the efficiency values. | Ostermeier et al. [31] | - | ✓ |