The PHA Depolymerase Engineering Database: A systematic analysis tool for the diverse family of polyhydroxyalkanoate (PHA) depolymerases

Table 1 Experimentally validated PHA depolymerases, which were used as seed sequences to set up the DED.

Accession number (gi)	Organism	Family	Reference
3641686	Ralstonia eutropha H16	Intracellular nPHA_SCL depolymerases (no lipase box)	[28]
75763431	Bacillus thuringiensis serovar israelensis ATCC 35646	Intracellular nPHA_SCL depolymerases (lipase box)	[35]
22035160	Rhodospirillum rubrum	Periplasmatic PHA depolymerases	[7]
130002	Pseudomonas oleovorans	Intracellular nPHA_MCL depolymerases	[18]
21689574	Pseudomonas putida		[17]
130019	Ralstonia pickettii	Extracellular dPHA_SCL depolymerises (catalytic domain type 1)	[29]
1777951	Alcaligenes faecalis		[23]
116744367	Bacillus megaterium		[48]
1730532	Paucimonas lemoignei		[20]
7385117	Paucimonas lemoignei		[31]
1657610	Paucimonas lemoignei		[15]
1621355	Paucimonas lemoignei		[15]
531464	Paucimonas lemoignei		[45]
531466	Paucimonas lemoignei		[45]
75538924	Pseudomonas stutzeri		[26]
5360565	Ralstonia pickettii
1381030	Ralstonia pickettii
4033618	Acidovorax sp. TP4	Extracellular dPHA_SCL depolymerises (catalytic domain type 2)	[25]
7209864	Caldimonas manganoxidans		[33]
565666	Comamonas sp.		[19]
75340123	Delftia acidovorans		[21]
47078657	Schlegelella sp. KB1a		[27]
1389770	Streptomyces exfoliatus		[24]
88192747	Penicillium funiculosum		[16]
15788987	Paucimonas lemoignei	Extracellular nPHA_SCL depolymerases	[6]
34452163	Pseudomonas alcaligenes	Extracellular dPHA_MCL depolymerases	[22]
29470160	Pseudomonas alcaligenes		[22]
21542177	Pseudomonas fluorescens		[30]

ISSN: 1471-2105