Figure 2

HRAS over-expression results in substantial relative isoform changes. (A) Example SplicerAV output comparing HRAS to GFP over-expression. Genes are ranked in order of descending Splice Score (top three genes shown), with EGFR receiving the top score in HRAS over-expression. Log₂ fold change in expression and corresponding p-values from two tailed homoskedastic t-test of differential expression are shown for individual probesets targeting each gene. Probesets are placed into A and B groupings by SplicerAV (see text). Splice score, SplicerAV p-value, and two way ANOVA p-values are shown for each gene. (B) Distribution of the 645 isoform changes (AS Events) predicted by SplicerAV (p < .01) upon HRAS over-expression in human primary mammary epithelial cells. For each gene, SplicerAV separates probesets into two similarly behaving groups based on similar fold changes in expression. The average change in expression between probesets in these two groups (AvgChange, see Equation 8 in methods) reflects the relative fold change in isoform abundance predicted by SplicerAV. Absolute relative fold change in isoform abundance is shown in log base 2.