Figure 4

Improving future sequencing efficiency using priors sequencing data. Example of the reporting possibilities. Run 1 had many unmappable and short, mapped sequences. Length distribution showed these were mainly 60-120 bp sequences. In Run 2 optimized PCR reactions and an additional length separation were carried out prior to the sequencing with a huge reduction (8% vs. 24%) of unmapped and short sequences and thus improving the cost-effectiveness.