Table 2 Specificity thresholds used in TOPSI runs for S. aureus
From: A high-throughput pipeline for the design of real-time PCR signatures
Specificity parameter | Strict threshold | Relaxed threshold |
|---|---|---|
M0 - longest stretch of contiguous matches with a non-target that has no mismatches | 18 | 18 |
M1 - longest stretch of contiguous matches with a non-target that has at most one mismatch | 20 | 40 |
M2 - longest stretch of contiguous matches with a non-target that has at most two mismatches | 22 | 40 |
M3 - longest stretch of contiguous matches with a non-target that has at most three mismatches | 24 | 40 |
Maximum overall identity with a non-target sequence | 90% | 90% |