Figure 8

Precise extraction of various shaped spots. Examples of the four shapes of fluorescence spots from micrograph are shown in the top row. (a) Gaussian-like shape (low peak height), (b) Gaussian-like shape (high peak height), (c) irregular shape, and (d) volcano shape. The 3-D maps of the original images and of the extracted spots using our proposed method are shown in the middle and bottom rows, respectively.