PeptideMine - A webserver for the design of peptides for protein-peptide binding studies derived from protein-protein interactomes

Table 3 Experimental validation of the peptides derived using PeptideMine as putative substrates of DLAR

Substrate peptide	Source Protein	Search method	V_max* (μ-mole/min/mg)	K_cat/K_m (sec^-1M^-1 ) × 10⁴
TRDI(pY)ETDYYRK	Insulin Receptor	Literature survey	24.31 ± 0.64	26.38 ± 0.04
TAEPD(pY)GALYE	Cuticle	Literature survey	26.48 ± 1.14	30.00 ± 0.07
CDDS(pY)FGNKC	Myospheroid	PeptideMine	13.86 ± 0.39	14.28 ± 0.05
VIGD(pY)VCRLCK	Nervous Fingers	PeptideMine	12.41 ± 0.68	19.59 ± 0.11
RDDT(pY)TAHAG	Abelson	PeptideMine	3.39 ± 0.19	17.96 ± 0.16

* Phosphatase activity was measured by conventional malachite green assays to detect the inorganic phosphate that is released, as the peptide is de-phosphorylated.

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