Figure 2From: Mutagenesis Objective Search and Selection Tool (MOSST): an algorithm to predict structure-function related mutations in proteinsExample multiple alignment of seven protein amino acid sequences. Each example protein has 16, 15, 15, 14, 15, 12 and 13 amino acids, respectively. The multiple alignment has a length of 18 positions, which means that every sequence has at least 2 gaps. The calculation of the mean and variance of the property Ω is shown in detail for position 9 of the alignment (i = 9), with 6 amino acids and one gap (n = 6: 1 histidine, 3 threonines and 2 serines).Back to article page