Table 4 LAMP signature candidates for Mycobacterium tuberculosis, with gene targets based on the reference H37Rv genome [GenBank: NC_000962.2]. The hyphen in FIP and BIP sequences represents where the two segments should be linked together
Signature | Size | Gene | Part | Sequence | Length |
|---|---|---|---|---|---|
mTub228 | 275 bp | Rv0987 | F3 | CGCTCTCAGTTTGATTGCCT | 20 bp |
| Â | Â | Â | B3 | CATTGCGAACATTGATGACA | 20 bp |
| Â | Â | Â | FLoop | CTCGGGTTACACCCAAACAG | 20 bp |
| Â | Â | Â | BLoop | CTTGATTGGAATTTGGCTCG | 20 bp |
| Â | Â | Â | FIP | GGATTCCGTCATTATCAGCCAAA-GACCGTTTTTCGCCATATTG | 43 bp |
| Â | Â | Â | BIP | CTGATTGGTACGGGCTTGGG-TGATGGTTTGAGTCACCAGG | 40 bp |
mTub229 | 195 bp | Rv0988 | F3 | AATGGCACCGCTTTGATG | 18 bp |
| Â | Â | Â | B3 | AATTTCGATTTCCCATGCAA | 20 bp |
| Â | Â | Â | FLoop | CTGGAAAATCGGGTCACACT | 20 bp |
| Â | Â | Â | BLoop | ATTTCCGCATCAAGACGACT | 20 bp |
| Â | Â | Â | FIP | CATCCCCGGCAACAAAGGTA-TTTTCAGATTCGACAGGCG | 39 bp |
| Â | Â | Â | BIP | GTGATCCCTCTCGAGTCGTCC-CACTCTGGTCACTGGTCCAA | 41 bp |
mTub230 | 252 bp | Rv1290c | F3 | GACACCACGATCAGCACG | 18 bp |
| Â | Â | Â | B3 | CGTAGTTCCGCCTAGTGCTC | 20 bp |
| Â | Â | Â | FLoop | GTCGATGATTCCCGTGAAAT | 20 bp |
| Â | Â | Â | BLoop | ACGGTTGAGCATGCTGGT | 18 bp |
| Â | Â | Â | FIP | AGTCTGGGTGCTGCCGACAT-GCCCTGAAACATCAGCTTGT | 40 bp |
| Â | Â | Â | BIP | CGCCCTGTAAGTAATCCAGTATGG-GGATGGGTGCATGCTTATTC | 44 bp |
mTub231 | 235 bp | tyrS (approx) | F3 | GATTGGGAGGTGATGAGACC | 20 bp |
| Â | Â | Â | B3 | CTAGTCGTGGGATACCAGCG | 20 bp |
| Â | Â | Â | FLoop | CGTACTCGATCCCGTTGTTC | 20 bp |
| Â | Â | Â | BLoop | TGCGTTGTAGTCGATTTCCA | 20 bp |
| Â | Â | Â | FIP | TCACACGCACAGCTGTTTAGTGA-TAGTGCGTTGACCTCACTCG | 43 bp |
| Â | Â | Â | BIP | CATCATCCTTTCATGTGACAGGC-CCGCCAAAGATAAGTCAGGA | 43 bp |
mTub232 | 230 bp | Rv2735c and recX | F3 | CGGTCTATGTTCTCGGGCT | 19 bp |
| Â | Â | Â | B3 | GTGGGTACGGCCAGACCT | 18 bp |
| Â | Â | Â | FLoop | GCCTTCAACAGGGCTAGTCA | 20 bp |
| Â | Â | Â | BLoop | CATCGAATCCCTTTAGACGC | 20 bp |
| Â | Â | Â | FIP | AACAAACTGGAGATACTTGCCGG-GTCGAGGTAAATTCGTTCACG | 44 bp |
| Â | Â | Â | BIP | CGCGTCCAATATGACCATTCTCTA-GTGGTTATCGCCGAGCTG | 42 bp |