From: pcrEfficiency: a Web tool for PCR amplification efficiency prediction
Univariate analysis | ||||||
---|---|---|---|---|---|---|
Variable | value | df | ρ | d | Log Odds | p-value |
Primers length | Z = -7.4398 | - | -0.118 | -0.239 | -0.433 | 1.008e-13* |
Sequence length | Z = -5.423 | - | -0.086 | -0.173 | -0.314 | 5.86e-08* |
Sequence G+C content | Z = -10.2664 | - | -0.163 | -0.331 | -0.601 | <2.2e-16* |
A repeats | Z = 2.1004 | - | 0.033 | 0.067 | 0.121 | 0.03569* |
T repeats | Z = 3.9818 | - | 0.063 | 0.127 | 0.230 | 6.84e-05* |
C repeats | Z = -5.294 | - | -0.084 | -0.169 | -0.307 | 1.196e-07* |
G repeats | Z = -7.1808 | - | -0.114 | -0.230 | -0.418 | 6.929e-13* |
Primers T m | Z = 1.4653 | - | 0.023 | 0.047 | 0.085 | 0.1428 |
Primers self complementarity | Z = 11.9002 | - | 0.190 | 0.386 | 0.700 | <2.2e-16* |
Primer dimers | Z = 4.4161 | - | 0.070 | 0.141 | 0.256 | 1.005e-05* |
Primer GC imbalance | Z = 11.1367 | - | 0.177 | 0.360 | 0.654 | <2.2e-16* |
Primers GC content | Z = 4.5921 | - | 0.073 | 0.147 | 0.266 | 4.388e-06* |
Sequence palindromes | Z = -3.4951 | - | -0.056 | -0.111 | -0.202 | 0.0004738* |
Species | χ2 = 585.616 | 9 | - | - | - | <2.2e-16* |
Template | χ 2 = 1241.562 | 12 | - | - | - | <2.2e-16* |
Variety or line | χ 2 = 585.8386 | 18 | - | - | - | <2.2e-16* |
Template source | χ 2 = 940.8915 | 24 | - | - | - | <2.2e-16* |
Operator | χ 2 = 727.4887 | 8 | - | - | - | <2.2e-16* |
Primer's 3' last two nucleotides | χ 2 = 237.911 | 15 | - | - | - | <2.2e-16* |