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Table 5 Near-full length sequences

From: Estimation of bacterial diversity using next generation sequencing of 16S rDNA: a comparison of different workflows

 

Skin axillary

Prairie soil

 

3%

5%

10%

3%

5%

10%

CROP

1009

1009

1009

1128

1128

1128

ESPRIT

59

43

26

504

340

162

MAFFT+JC

47

39

39

490

346

183

MAFFT+MAFFT

266

126

109

1007

895

714

MAFFT+Mothur

50

39

36

503

351

181

Mothur+JC

47

39

36

491

352

184

Mothur+MAFFT

269

126

109

977

872

689

Mothur+Mothur

54

40

38

535

396

204

Mothur+PreC+ JC

47

39

36

491

353

268

Mothur+PreC+MAFFT

287

127

109

977

877

836

Mothur+PreC+Mothur

55

40

38

536

397

305

MUSCLE+JC

50

39

37

480

337

175

MUSCLE+MAFFT

266

126

109

1007

895

714

MUSCLE+Mothur

58

40

38

487

339

173

Otupipe

49

37

26

490

336

160

RDP

60

43

27

504

348

175

  1. OTUs observed with each of the workflows analysed at distances of 3, 5 and 10% for the skin axillary microbiome data described by Hao et al. [23], and tall grass prairie soil data [37].