An efficient algorithmic approach for mass spectrometry-based disulfide connectivity determination using multi-ion analysis

Table 3 DMS and FMS mass space sizes comparison

Protein	Disulfide Bond	Full Search (exponential)		Proposed Search (polynomial)		DMS decrease	FMS decrease
DMS size	FMS size	DMS size	FMS size
Beta-LG	C⁸²C¹⁷⁶	2152	2169	1870	78	13.1%	96.4%
ST8Sia IV	C¹⁴²C²⁹²	1246	1792	1038	106	16.7%	94.1%
C¹⁵⁶C³⁵⁶	1246	2640	1038	255	16.7%	90.3%
FucT VII	C³¹⁸C³²¹	581	115	528	34	9.1%	70.4%
C⁶⁸C⁷⁶	879	103	681	41	22.5%	60.2%
C²¹¹C²¹⁴	879	1819	681	107	22.5%	94.1%
B1,4-GalT	C¹³⁴C¹⁷⁶	2149	1189	1127	77	47.6%	93.5%
C²⁴⁷C²⁶⁶	2149	5480	1127	426	47.6%	92.2%
Average DMS and FMS decrease						21.8%	86.4%

ISSN: 1471-2105