Figure 4

PCR experimental validation of the detected EEJ, NM_007108 (4:2-4). The marker lane is labeled with M and is presented as 50bp ladder. The central bright band in the marker lane is equal to 350 bp. (a) UCSC Genome Browser shows that four ESTs match the detected EEJ. (b, c) The PCR result showed that the expected EEJ (d transcript) can be experimentally detected. c and d denotes continuous and discrete transcripts, respectively. (d) The sequencing result provided the authenticity of the detected EEJs.