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Table 7 Comparisons between searches with different portions of unidentifiable spectra

From: False discovery rates in spectral identification

Search#

Spectra

Database

PMTol

# spec

EmpiricalFDR fixed

FactFDR fixed

     

N target

FactFDR(%)

p-value(%)

N target

EmpiricalFDR(%)

I-1

ISB-02

ISB

2.5 Da

4,966

2329/1009

5.8/4.4

10.9/30.9

2279/1024

3.9/5.7

I-14

ISB-02+AB-TC

ISB

2.5 Da

11,285

2262/984

5.8/4.5

5.7/34.5

2221/995

4.0/6.0

I-3

ISB-02

ISB+Yeast

2.5 Da

4,966

1578/602

4.7/5.1

40.8/50.3

1583/596

5.1/3.9

I-5

ISB-02+AB-TC

ISB+Yeast

2.5 Da

11,285

1490/569

5.0/5.8

50.2/31.2

1480/553

4.5/4.3

I-15

ISB-02+AB-All

ISB+Yeast

2.5 Da

24,948

1367/531

4.2/5.3

33.0/34.5

1393/518

5.5/4.2

Y-1

Y-Small+AB-TC

Yeast+AT

30 ppm

16,077

2574/1988

1.0/1.3

50.1/22.7

2588/1759

1.0/0.5

Y-6

Y-Small+AB-All

Yeast+AT

30 ppm

29,740

2238/1913

1.1/1.4

44.2/14.7

2208/1629

0.9/0.7

  1. Adding unidentifiable spectra reduces the number of positive PSMs, but does not change the accuracy of FDR estimations significantly.