Figure 2

Induction curves for the HSL-inducible system in different copy number contexts. The HSL-inducible device (HSL _RFP ) was characterized in the exponential phase after at least 50 minutes from the induction in a microplate reader. For each copy number condition, the curve is expressed in absolute arbitrary units of S _cell (left vertical axis) and in RPUs (right vertical axis), computed as the ratio between the S _cell of the inducible device and the S _cell of the J23101 promoter (via the 101 _RFP measurement device) in the same growth and copy number condition, considering the same reporter gene (RFP). Experimental data were fitted with a Hill function (continuous line) and the estimated parameters are reported in the boxes with their coefficients of variation. Error bars represent the 95% confidence intervals of the mean value (circles) computed on 3 clones.