Figure 2

Junction Accuracy of PASTA and other software. The two panels display the results of the comparison of PASTA with other junction detection pipelines on simulated datasets. In the first simulation, the frequencies of indels, substitutions and sequencing errors were 0.05%, 0.1% and 0.5% respectively, and 80% of the splice signals were from annotated splice isoforms. In the second simulation, the frequencies of indels, substitutions and sequencing errors were 0.25%, 0.5% and 1% respectively, with 65% of the splice signals coming from annotated splice forms. In addition, 25% of the trailing 10 bases are subject to a 50% sequencing error rate. Reproduced with permission from [4].