Figure 5

Using transfer functions to visualize features seen in standard histology. A surgical resection of a breast biopsy was imaged using IR spectroscopy and then stained and imaged with a standard optical microscope. (a) A section of the hematoxylin and eosin (H&E) stained tissue is shown. The proposed techniques can be used to selectively visualize various structural and chemical features using only the IR hyperspectral image. (b) Scattering effects are highlighted, indicating regions containing highly scattering structures (edges and collagen fibers). (c) Removal of scattering features allows the selection of tissue thickness based on the absorbance of Amide I (1650cm ⁻¹). (d) Using Amide I as a reference, chemical compounds corresponding to epithelial cells are visualized using the centroid of the Amide II band (≈1550cm ⁻¹). (e-f) The corresponding joint histograms are shown, with selected pixels highlighted.