Table 1 Overall run performances
From: Routine performance and errors of 454 HLA exon sequencing in diagnostics
Run | Â | 1 | 2 | 3 | 4 | 5 | 6 |
|---|---|---|---|---|---|---|---|
Passed Filter | [reads] | 58,303 | 58,230 | 59,991 | 70,988 | 70,477 | 55,803 |
Short | [%] | 48.97% | 51.79% | 45.68% | 44.29% | 42.92% | 54.74% |
Qual 98% 400 bp | [%] | 82.68 | 80.61 | 85.18 | 76.49 | 82.37 | 75.84 |
Control | [wells] | 5,688 | 4,626 | 5,701 | 5,117 | 7,471 | 6,084 |
Raw | [wells] | 169,261 | 187,287 | 163,378 | 167,569 | 180,379 | 187,583 |
Key pass | [wells] | 161,395 | 180,717 | 155,583 | 160,344 | 170,507 | 178,334 |
Median Read length | [bp] | 417 | 418 | 424 | 414 | 316 | 406 |
Avg Read length | [bp] | 408.94 | 410.24 | 411.66 | 402.70 | 349.29 | 379.03 |
mixed | [%] | 10.89% | 11.36% | 10.49% | 7.73% | 10.85% | 10.80% |
dot | [%] | 3.82% | 4.43% | 5.04% | 3.53% | 4.69% | 2.87% |
Bases | [Mb] | 24.31 | 24.34 | 25.44 | 29.39 | 22.27 | 22.66 |