Table 1 Summary of probe and design variables and hybridization intensities for eight arrays
Variable | Minimum value | Maximum value | Median value |
|---|---|---|---|
Raw intensity | 44 | 65530 | 395 |
Background intensity | 26 | 653.5 | 36 |
Positive control intensity | 70 | 15103 | 9346.75 |
Negative control intensity | 39 | 135 | 57.5 |
Probe GC (%) | 11.67 | 86.67 | 36.67 |
Free energy (kcal/mol) | -9.48 | 3.8 | 0.48 |
Off-target matches | 0 | 4496 | 0 |
PolyA length (bp) | 0 | 19 | 3 |
PolyC length (bp) | 0 | 17 | 0 |
PolyG length (bp) | 0 | 19 | 0 |
PolyT length (bp) | 0 | 19 | 3 |
Segment length (bp) | 61 | 5760 | 1156 |
Distance (bp) | 6 | 7791 | 190.5 |
Offset (bp) | 6 | 26 | na |
Probe position | 1 | 389 | na |