Figure 4

Phenotypic map of the Mitocheck screen. Phenotypic profiles of all siRNA treatments were derived from fitted parameters and projected into a two dimensional space using linear discriminant analysis between the control spots siScrambled, siCOPB1 and siKIF11. Color contour lines denote the quantiles of control experiments that fall into the corresponding phenotypic region. The green phenotypic region, centered on the non-targeting negative control siScrambled, is representative of a normal growth phenotype. The orange phenotypic region, centered on siKIF11, is representative of prometaphase arrest followed by cell death, while the blue region, centered on siCOPB1, is representative of cell death without mitotic arrest. Shown are the 2190 siRNAs that induced a disruption of the cell cycle, or increased the duration of the interphase or mitosis phases. A selection of siRNA gene targets are highlighted on the map.