Figure 3From: ZebIAT, an image analysis tool for registering zebrafish embryos and quantifying cancer metastasisSegmentation of a DIC image. Top left: grayscale image with inverted grayscale values. Top right: Image filtered with LoG. Middle left: Result from LoG edge detection. Middle right: Result from Otsu's threshold. Bottom left: Segmented image after all steps, including morphological closing. Bottom right: Outline of the fish.Back to article page