Figure 4

Segmentation and tracking of mycobacterial cells using BactImAS. Cell segmentation results are presented as red (automated) and green (manual) outlines with phase-contrast images (top row) and by shaded colors (bottom row) at indicated times. In the bottom row, different stages of descent from a common ancestor (generation) are represented by different nuances of red and manual interventions as white cells. Scale bar, 5 μm.