Figure 5

Detecting genome amplification events. Depth of coverage data for all 14 chromosomes of a Dd2 clonal line with evolved resistance to cladosporin (CladoR_clone3) [10]. The curve represents the output of the CNV detection algorithm, while the black line is the actual copy number call by the program. The two visible amplifications detected are the one encompassing the pfmdr1 locus on chromosome 5 and the amplification associated with cladosporin-resistance on chromosome 13 [10]. A third amplification on chromosome 12 containing pfgch is visible at higher magnification. The inset shows a read pileup for the region containing an amplification on chromosome 13. The green colored reads indicate those with an abnormal insert size with mated reads mapping back-to-back rather than front-to-front. This is evidence that the amplification is in tandem with the original copy on the chromosome.