Figure 6

Creation of a new collection, instrument control and data-acquisition. A) Dialog to create a new collection. Importantly, a collection protocol (1) must be chosen, and an importer can be selected if needed (2). B) Example workflow for biological experiments and single cell “lyse and spread” visual proteomics. C) openBEB plug-ins employed to control the instrument, perform time-lapse imaging of the cells, prepare samples from individual cells for EM (i.e., dialysis/staining and deposition on EM grid) and subsequently import EM images for analysis by visual proteomics. D) Tree-like structure of collections containing datasets and metadata created by openBEB. The relationship between the workflow and openBEB components is indicated by red arrows (3). The blue arrows (4) show the relationship between the openBEB components and the collection-tree. Note that the cell culture LM images define a coordinate system, and the coordinates of subsequent EM images define a sub-coordinate system (HC system, see text). Instrument control and data acquisition plug-in combining LM and single cell lysis. This plug-in contains different modules communicating via the openBEB macro system. (5) Live cell imaging. (6) Stage control. (7) Control optics. (8 to 10) Pump-system and electroporation control.